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2 edition of Protein interactions in Fanconi anemia. found in the catalog.

Protein interactions in Fanconi anemia.

Susan M. Gordon

Protein interactions in Fanconi anemia.

by Susan M. Gordon

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Published .
Written in English


About the Edition

Fanconi anemia (FA) is a genetically heterogeneous disorder characterized by bone marrow failure, cancer predisposition, and increased cellular sensitivity to DNA-crosslinking agents. Protein products of seven of the nine FA genes identified thus far participate in a protein complex required for monoubiquitination of the FANCD2 protein. This thesis characterizes protein interactions that contribute to the architecture of this FA protein complex as well as its connection to the downstream FA pathway component FANCD2. The yeast two-hybrid system is used to identify and map the contact points of direct FANCA-FANCG, FANCF-FANCG, FANCC-FANCE and FANCD2-FANCE binding, and to assess the impact of patient-derived missense mutations on the integrity of these interactions.Given the ability of FANCG and FANCE to interact directly with multiple FA proteins, their ability to further contribute to complex assembly by mediating interactions between complex components was tested in the yeast three-hybrid system. FANCG was able to mediate interaction of FANCA with FANCF as well as between monomers of FANCA, suggestive of a role in multiple stages of complex assembly. FANCE was able to mediate interaction of FANCC with FANCF, a complicated association given that FANCF interacted with neither FANCC nor FANCE in the two-hybrid system.The ability of FANCE to mediate an interaction between FANCC and FANCD2 was also demonstrated in the yeast three-hybrid system and the association of FANCC with FANCD2 was further confirmed in human cells. Formation of the FANCC/FANCE/FANCD2 ternary complex was reduced or absent in cell lines derived from patients of most FA complementation groups, and was rescued in FA-E cells by exogenous expression of wild-type FANCE. Yeast two-hybrid screening of a library of randomly mutagenized FANCE constructs identified FANCE mutants capable of interacting with FANCC but not with FANCD2. Exogenous expression of these mutants in a FA-E cell line demonstrated an absolute requirement for the FANCE/FANCD2 interaction in maintaining the integrity of the FA DNA-damage response pathway. Thus FANCE was demonstrated to be a key mediator of protein interactions, both in assembly of the FA protein complex and in connection of complex components to the downstreamtargets of complex activity.

The Physical Object
Pagination151 leaves.
Number of Pages151
ID Numbers
Open LibraryOL21302915M
ISBN 100494077441

Protein-protein interaction databases BioGrid i: , 61 interactors: CORUM i: Q DIP i: DIPN: IntAct i Q protein Structure i 3D structure databases Fanconi anemia group C protein. FANCC. Annotation score: B1ALR7: B1ALR7_HUMAN: Fanconi anemia group C protein. Taniguchi T, D'Andrea AD () The Fanconi anemia protein, FANCE, promotes the nuclear accumulation of FANCC,Blood, (7), Taniguchi T, Garcia-Higuera I, Andreassen PR, Gregory RC, Grompe M, D'Andrea AD () S-phase-specific interaction of the Fanconi anemia protein, FANCD2, with BRCA1 and RAD51, Blood, (7),

Fanconi Anemia (FA) is a rare autosomal genetic disease with multiple birth defects and severe childhood complications for its patients. The lack of sequence homology of the entire FA Complementation Group proteins in such as FANCC, FANCG, FANCA makes them extremely difficult to characterize using conventional bioinformatics methods. A novel BTB/POZ transcriptional repressor protein interacts with the Fanconi anemia group C protein and PLZF Maureen E. Hoatlin, Zhi Yu, Helen Ball, Kirsten Silvey, Ari Melnick, Stacie Stone, Sally Arai, Nicola Hawe, Gareth Owen, Arthur Zelent, Jonathan D. Licht.

Title: Fanconi Anemia Proteins, DNA Interstrand Crosslink Repair Pathways, and Cancer Therapy VOLUME: 9 ISSUE: 1 Author(s):Paul R. Andreassen and Keqin Ren Affiliation:Cincinnati Children's Research Foundation, Burnet Ave. ML S, Cincinnati, OH , USA. Keywords:Fanconi anemia, DNA interstrand crosslinks, homologous recombination, DNA repair, DNA damage . A novel BTB/POZ transcriptional repressor protein interacts with the Fanconi anemia group C protein and PLZF Maureen E. Hoatlin, Zhi Yu, Helen Ball, Kirsten Silvey, Ari Melnick, Stacie Stone, Sally Arai, Nicola Hawe, Gareth Owen, Arthur Zelent, Jonathan D. LichtCited by:


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Protein interactions in Fanconi anemia by Susan M. Gordon Download PDF EPUB FB2

DNA damage activates the monoubiquitination of the Fanconi anemia Protein interactions in Fanconi anemia. book protein, FANCD2, resulting in the assembly of FANCD2 nuclear foci.

In the current study, we characterize structural features of FANCD2 required for this intranuclear by: Fanconi anemia is a chromosomal breakage disorder with eight complementation groups (A–H), and three genes (FANCA, FANCC, and FANCG) have been l investigations of the interaction between FANCA and FANCC, principally by co-immunoprecipitation, have proved by: Introduction.

Fanconi anemia (FA) is an autosomal recessive cancer susceptibility syndrome characterized by multiple congenital anomalies and progressive bone marrow failure (reviewed in Huibregtse et al., ) (D'Andrea and Grompe, ).FA patients develop several types of cancers including acute myeloid leukemias and squamous cell carcinomas of the head and neck (Alter, ).Cited by: CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): Fanconi anemia (FA) is a rare autosomal recessive disease characterized by skeletal defects, anemia, chromosomal instability and increased risk of leukemia.

At the cellular level FA is characterized by increased sensitivity to agents forming interstrand crosslinks (ICL) in DNA. Protein–protein interactions of Fanconi anemia proteins FANCI, FANCD2 and BRCA2 Conference Paper in Acta Crystallographica Section A: Foundations. Fanconi anemia (FA), a cancer predisposition syndrome exhibits hallmark feature of radial chromosome formation, and hypersensitivity to DNA crosslinking agents.

A set of FA pathway proteins. A High-Throughput Screening Strategy to Identify Protein-Protein Interaction Inhibitors That Block the Fanconi Anemia DNA Repair Pathway Andrew F.

Voter, Kelly A. Manthei, and James L. Keck Journal of Biomolecular Screening 6, Cited by:   Proteins of the Fanconi anaemia pathway are master regulators of genomic integrity through their interactions with other DNA repair pathways to Cited by: Fanconi Anemia: A Handbook for Families.

questions relating to treatment or prognosis, please raise these issues with your doctor or with an appropriate specialist. The Fanconi Anemia Research Fund recently published Fanconi Anemia: Standards for Clinical Care, a handbook for treating physicians.

Copies are available from the FARF office. Fanconi Anemia Guidelines for Diagnosis and Management 4 Consequently, early involvement with a major transplant center experienced in FA transplants and with a multi-disciplinary consultation team is optimal.

FA is a cancer-prone disorder. • Close monitoring, especially. Seventy-five years ago, Dr. Guido Fanconi reported three siblings who exhibited both congenital defects and aplastic anemia.1 Since then, we have learned that Fanconi anemia (FA) is a rare multigenic disorder (a prevalence of per million2), that predisposes children and adults to life-threatening bone marrow failure, myelodysplasia,3 acute nonlymphocytic leukemia (AML), and certain Author: Grover C.

Bagby. Figure 1. Yeast two-hybrid analysis of BRCA2 interactions with FANCG. (A) Bars represent tested GAL4 BD-BRCA2 fragments, with numbers referring to amino acid position within the BRCA2 coloured red represent BRCA2 fragments which showed strong interaction with FANCG, yellow colouring represents a moderate interaction and uncoloured bars represent no interaction Cited by: Induction of the Fanconi anemia (FA) DNA repair pathway is a common mechanism by which tumors evolve resistance to DNA crosslinking chemotherapies.

Proper execution of the FA pathway requires interaction between the FA complementation group M protein (FANCM) and the RecQ-mediated genome instability protein (RMI) complex, and mutations that disrupt FANCM/RMI interactions Cited by: FANCI:FANCD2 monoubiquitination is a critical event for replication fork stabilization by the Fanconi anemia (FA) DNA repair pathway.

It has been proposed that at stalled replication forks, monoubiquitinated-FANCD2 serves to recruit DNA repair proteins that contain ubiquitin-binding motifs. Here we have reconstituted the FA pathway in vitro to study functional consequences of.

Regulated interaction of the Fanconi anemia protein, FANCD2, with chromatin. The weaker or transient interactions of FANCE with both FANCA and FANCG could provide a temporary link between FANCC and the main FA protein complex.

Again, these novel interactions suggest that the protein encoded by the newly described FANCE gene is intimately connected to the main FA by:   The ID complex, involving the proteins FANCI and FANCD2, is required for the repair of DNA interstrand crosslinks (ICL) and related lesions 1.

These proteins are mutated in Fanconi anaemia Author: Renjing Wang, Shengliu Wang, Ankita Dhar, Christopher Peralta, Nikola P. Pavletich. Fanconi anemia (FA) is a rare genetic disorder characterized by aplastic anemia, cancer/leukemia susceptibility and cellular hypersensitivity to DNA crosslinking agents, such as cisplatin.

To date, 12 FA gene products have been identified, which cooperate in a common DNA damage-activated signaling pathway regulating DNA repair (the FA pathway). The Fanconi anemia (FA) core complex is the ~mDa ubiquitin ligase most frequently mutated in patients with FA.

New cryo-electron microscopy (cryo-EM) data from Shakeel et al. reveals a surprisingly complex ubiquitin ligase architecture, providing unprecedented insight into this critical hub at the interface of DNA crosslink detection and : Jacob D. Aguirre, Nicolas H. Thomä. Plays an essential role in the repair of DNA double-strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites.

Required for maintenance of chromosomal stability. Specifically binds branched DNA: binds both single-stranded DNA (ssDNA) and double-stranded.

Fanconi anemia (FA) is an autosomal recessive syndrome characterized by progressive bone marrow failure and cancer predisposition. Eight FA complementation groups have been identified. The FANCA, FANCC, FANCE, FANCF, and FANCG proteins form a nuclear complex required for the monoubiquination of the FANCD2 by: Fanconi anaemia, complementation group A, also known as FAA, FACA and FANCA, is a protein which in humans is encoded by the FANCA gene.

It belongs to the Fanconi anaemia complementation group (FANC) family of genes of which 12 complementation groups are currently recognized and is hypothesised to operate as a post-replication repair or a cell cycle s: FANCA, FA, FA-H, FA1, FAA, FACA, FAH. Fanconi anemia group F protein is a protein that in humans is encoded by the FANCF gene.

Interactions. FANCF has been shown to interact with Fanconi anemia, complementation group C, FANCG, FANCA and FANCE. Function. FANCF is an adaptor protein that plays a key role in the proper assembly of the FA core complex.

The FA core complex is composed.